Nucleic Acid (DNA/RNA) Extraction Kit
1. Introduction Nucleic Acid Extraction Reagent Kit,Nucleic Acid Extraction Kit,Covid-19 Nucleic Acid Extraction Reagents,Nucleic Acid Test Kits Jilin Sinoscience Technology Co. LTD , https://www.jlgkscience.com
The total viral nucleic acid extraction kit is suitable for extracting total viral nucleic acid from serum, plasma, tissue homogenate and other samples. The kit is based on silica column purification technology, which eliminates the need for toxic phenol-chloroform extraction and time-consuming alcohol precipitation. This product has successfully extracted nucleic acids from hepatitis B A/C, hepatitis C, and norovirus standard. The obtained DNA/RNA can be directly used in a series of downstream experiments such as PCR, RT-PCR, and LAMP.
Notice:
1. The carrier RNA solid must be dissolved in Nuclease Free Water to 1µg/µl before use, and vortex to dissolve. Store in aliquots at -70°C. If you need to store it at -20℃ for a long time, please repackage it according to the number of times of use.
2. Dissolve Proteinase K (20mg/ml): Add Proteinase Dissolve Buffer to dissolve Proteinase K to a final concentration of 20mg/ml. Proteinase K dry powder can be stored at 2-8°C for one year, but dissolved Proteinase K must be stored in aliquots at -20°C. Repeated freezing and thawing of Proteinase K can affect its activity.
3. Buffer VHB must be diluted with 14 ml absolute ethanol before use and stored at room temperature.
4. Buffer RW2 must be diluted with 80 ml of absolute ethanol before use and stored at room temperature.
3. Shelf life
Except for Proteinase K and Carrier RNA, other components of this product can be stored at room temperature (15-25°C) for 12 months, and should be stored at 2-8°C for long-term storage. Proteinase K and Carrier RNA dry powder are transported at room temperature. Please store at -20°C after receiving the test product, and store at -20°C after dissolving.
1. Choose High-Quality Seeds
When planting winter soybeans, it's essential to select varieties that are upright in growth, resistant to lodging, shading, and pests, and have a short growing season. Varieties like Zhongpin 661 are highly recommended due to their strong yield potential, good fertility, and adaptability to colder conditions. Using high-quality seeds ensures better germination, stronger seedlings, and higher productivity.
2. Sow at the Right Time
Sowing time is critical for winter soybean cultivation. The growing period typically lasts around 110 days, so it's best to sow in mid to late November or early December. Sowing too early can expose the plants to cold stress during flowering, while sowing too late may lead to difficulties in harvesting due to rain. It’s also important to rotate crops properly, such as after rice, corn, or flue-cured tobacco. Before sowing, ensure the seeds are of high quality to promote healthy emergence, strong seedlings, and uniform growth.
3. Apply Fertilizers Appropriately
Applying the right amount of fertilizer at the right time is crucial for optimal growth. Basal fertilization should provide essential nutrients for early growth, with a focus on organic materials and balanced nitrogen, phosphorus, and potassium. A typical recommendation is 10–15 kg of compound fertilizer per acre. At the early flowering stage, applying 3–5 kg of urea per mu helps boost flower production. Foliar sprays with 2% superphosphate and 0.05% ammonium molybdate, or 0.1% borax solution, can enhance grain filling and improve yield.
4. Strengthen Field Management
Proper field management is key to achieving high yields.
(1) After seedlings emerge, inspect the field and promptly transplant any weak or submerged seedlings to ensure uniform growth. During the seedling stage, encourage early development by promoting branching and root establishment. In well-fertilized soils, pinching off the growing tip when the plant has 4–6 true leaves can help control excessive vegetative growth. For winter soybeans, using growth regulators like paclobutrazol or chlorhexidine at 5–8 true leaves can reduce legginess, increase pod numbers, and improve grain fill. Regular weeding, soil cultivation, and mulching help maintain soil structure, moisture, and microbial activity, which support healthy root development. Common pests during this stage include underground insects and aphids. If no pre-planting pesticide was used, apply 500 ml of 50% phoxim EC mixed with 50 kg of water per acre, or spray 40% dimethoate EC at 1000x dilution to control aphids.
(2) During the flowering stage, both vegetative and reproductive growth occur simultaneously. This is a critical time for nutrient and water supply, as insufficient resources can lead to flower drop and reduced yield. Ensure proper irrigation and apply 5–7.5 kg of urea per acre at the start of flowering to promote more flowers and pods. Excessive moisture can cause tall, weak plants with poor ventilation, leading to flower drop and lodging. In such cases, chemical growth regulators like paclobutrazol or potassium triiodobenzoate can be used. If the soil is rich and plants are vigorous, additional fertilizer may not be necessary, but top-dressing combined with weeding can still be beneficial.
(3) Grain Filling Stage
This phase starts when the pods begin to swell and continues until they reach full size. During this time, the plant transfers most of its nutrients to the grains. To ensure maximum yield, monitor for signs of premature aging and apply foliar sprays of 0.3% urea, 0.3% potassium dihydrogen phosphate, and 0.05% ammonium molybdate, with 0.3% detergent added to improve coverage. If drought occurs, apply “granule water†to boost yield and quality. As the grains mature, reduce soil moisture to speed up ripening. Common diseases during this stage include purple spot, gray spot, and borer disease. Use 50% carbendazim WP at 500 times dilution, starting at the first sign of disease and repeating every 7 days. For borer control, spray 2% acacia pine powder or 50% agarose-killed pine oil, focusing on the middle and upper pods.