For Chinese sturgeon farming Preparation of Microecological Preparation and Evaluation of Its Application Effect (Zhang Yaohong 1 Cao Jieying 2 Yuan Chunying 3 Kang Chenxiang 2 Ge Jing 1 Wang Zhenhuai 4) (1 - Baoding Fisheries Research Institute 2 - Hebei Fisheries Technology Extension Station 3 - Tianjin University of Science and Technology 4 - Zhangzhou Fishery Technology Promotion Station) The current culture of Chinese sturgeon has its unique features: the temperature of the culture water is constant between 30°C and 2°C; the breeding space is relatively closed; the aquaculture capacity is relatively large; all feeds are high-protein, high-fat feed, and feed residue and metabolites contain Higher organic nitrogen and so on. These features constitute the algae and fungi that are compatible with them. For this purpose, we screened and identified high-efficiency degrading bacteria from the sludge and pool water of the Chinese sturgeon aquaculture pond; and the mass production was carried out by the Chuzhou Wangchang Institute of Materials Technology using modern technology, and was carried out in the city of Fuping Yangshuo. Test promotion has achieved good results. 1. Development of micro ecological preparations 1.1 Screening of degrading bacteria Dianchi effluent and pool water samples were randomly taken into sterilized raw water samples and cultured for 3 cycles at 30°C. The cultures were then plated on selective media and colonies were selected for further culture. Inoculated in the raw water-like medium, a TOC sample was taken after 24 hours, and strains with significant TOC degradation were screened for further performance testing and identification. 1.2 Preparation of Microecological Preparations Based on the identification results, the Bozhou State Matter Technology Research Institute expands the culture with an exclusive culture medium and uses a modern process to solidify a micro ecological preparation containing a total number of living bacteria of 8 billion/g and rich in a variety of active enzymes. 2. Application test of probiotics 2.1 Test site and basic conditions Select the No. 2# pool, No. 3 pool and No. 4 pool in the 1st row of Fupingshanquan special breeding farm as test sites. The area of ​​the three ponds is 330m2. Each pond is stocked with 35g quail species of 4000 and fed by Hebei Normal University. "Kangtai" brand Chinese sturgeon special feed, daily bait rate of 3-3.5%; test water temperature 30 °C 2 °C, water depth 60cm. 2.2 Test methods A control experiment was used in which 3# was used as a control pool, 2# pool and 4# pool as a test pool. At 11 o'clock on August 15, 2005, No. 2 pool was sputtered with the developed micro-ecological preparation (code I, hereinafter referred to as No. I), and No. 4 pool was used to sprinkling the universal micro-ecological preparation produced by Wang Miao Technology Research Institute. (Code II, hereinafter abbreviated as II), the water chemical indicators, microbiological indicators, and plankton biomass were measured at regular intervals, and the growth of the pups was measured at the end of the experiment, and internal organs were dissected and observed. 2.2.1 Determination of the pH value of water chemistry using a pH meter; COD using the acid potassium permanganate method; NH4+-N using Nessler's colorimetric method; NO2 - N using naphthylamine hydrochloride colorimetry; NO3 - the use of phenol II Sulfonic acid colorimetric method; DO using iodometric method (measured as early as 4:00); transparency using Saskatchewan disk determination; total phosphorus using molybdate amine colorimetry; hydrogen sulfide using iodometric method. 2.2.2 Classification of bacterial flora 2.2.2.1 Collection and treatment of samples Collect water samples in a sterilized fine-mouth bottle. Pipette a small sample with a sterile pipette. Use sterile water for 10 times serial dilution in a sterile test tube. Take 103, 104 times dilution water. Sample count. 2.2.2.2 Bacterial Classification Counts The number of bacteria in the five genera of Aeromonas sp., Vibrio, Bacillus, Salmonella, and Escherichia were determined. The corresponding selective medium for each genus was selected and poured into sterilized petri dishes of the same size. 0.5 ml of the diluted water sample was then applied to a sterile petri dish. Each genus served as a parallel 2 petri dish. After 24 to 28 h incubation at 30°C, the number of plaques on the plates was directly counted. The average value of the two parallel plates was the number of bacteria in the genus. 2.2.2.3 Determination of planktonic biomass Mix equal amounts of water in the middle and lower parts of each pool, reference the method for surveying plankton resources, fix and concentrate, and use the counting box to count the phytoplankton and zooplankton in each pool. analysis. 2.2.2.4 Measurement of Biological Indexes The body width, body thickness, body weight, skirt width and other indicators of the calf are mainly measured, and the body ratio coefficient and the skirt coefficient are calculated. 2.3 Results 2.3.1 Effects of Microecological Preparations on Water Chemical Indicators Table 1 shows the measurement results of water chemistry for 1-15 days after the control tanks and the test tanks were splashed with micro ecological preparations. It can be seen from Table 1 and Figures 1-4 that NH 2 +-N, NO 2 -N, NO 3 -, COD, and hydrogen sulfide gradually decrease in 2 to 15 days, DO increases gradually, and 4# changes in 1-10 days. Same as 2#, but NH4+-N, NO2-N, COD, and H2S in pond 15d4 have slightly recovered, DO decreases slightly; PH and total phosphorus do not change much during the whole experiment, and the water in control pond Changes in chemical indicators were not significant. Table 1 Comparison of chemical indicators of the control and test pools index Groups Control pool 3# Test Pool 2# Test pool 4# 1d 5d 10d 15d 1d 5d 10d 15d 1d 5d 10d 15d PH 8.1 7.9 7.8 8.0 8.0 8.1 7.8 8.0 8.0 7.9 8.0 8.0 Dissolved oxygen (mg/L) 4.76 4.65 4.87 4.69 4.80 5.63 5.77 6.01 4.73 5.48 5.69 5.30 Ammonium nitrogen (mg/L) 0.57 0.60 0.59 0.59 0.58 0.34 0.35 0.29 0.57 0.38 0.37 0.43 Nitrous Nitrogen (mg/L) 0.09 0.10 0.09 0.11 0.10 0.03 0.01 0.01 0.10 0.04 0.03 0.05 Nitrate Nitrogen (mg/L) 0.11 0.12 0.10 0.12 0.12 0.08 0.02 0.03 0.11 0.07 0.05 0.06 Organic oxygen consumption (mg/L) 12.7 13.3 13.5 14.1 13.1 11.8 9.7 9.3 12.9 11.6 9.9 10.2 Total phosphorus (mg/L) 2.45 2.52 2.44 2.49 2.51 2.52 2.50 2.49 2.65 2.26 2.49 2.58 Hydrogen sulfide (mg/L) 0.3 0.25 0.3 0.4 0.3 not found out not found out not found out 0.2 not found out not found out 0.1 Fig. 1 Changes in dissolved oxygen during the test Fig. 2 Changes of nitrate nitrogen during the test Fig. 3 Changes of nitrite nitrogen during the test Figure 4 Changes in organic oxygen consumption during the test period 2.3.2 Effects of Microecological Agents on Changes in the Number of Major Microflora in Aquatic Rearing Water The effects of microecological preparations on the number of main bacteria in the rearing water bodies are shown in Table 2. As can be seen from Table 2 and Figures 5-7, the amount of Aeromonas, Vibrio, Salmonella, and Escherichia coli gradually decreased with the passage of time while the number of Bacillus increased steadily. The number of bacteria in the control pool did not change much. Table 2 Number of bacterial populations in control and test pools index Groups Control 3# pool Test Pool 2# Test pool 4# 1d 8d 15d 1d 8d 15d 1d 8d 15d Aeromonas (Aromonas) 63 64 62.5 64 30 12 61 34 27 Vibrio (Vibrio) 6 6.5 6 6.5 Chongqing "Terson" Coptis", Department of Chongqing Shizhu Tujia Autonomous County specialty, China National Geographic Indications (native geographical products). Produced in Shizhu County Huangshui National Forest Park, also known as "Coptis". 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