Introduction to the main methods of space disinfection in clean areas

Overview:

Generalized space disinfection includes: space disinfection and space sterilization. Narrow space disinfection refers to the method of killing pathogenic microorganisms in a certain space, but not necessarily killing bacterial spores. Chemical methods are often used to achieve disinfection. Chemicals used for disinfection are called disinfectants. Space sterilization refers to the method of killing all microorganisms (including bacterial spores) on the object, usually by physical means to achieve the purpose of sterilization. The technique of preventing the entry of microorganisms into the body or object is called aseptic operation.

Definition of space disinfection:

Space Disinfection refers to the use of physical and chemical methods to kill microorganisms or inhibit the growth of microorganisms in a certain space.

Space disinfection objects:

The object of space disinfection is mainly microorganisms in space. Microorganisms are tiny organisms that are invisible or invisible to the naked eye. They are small, simple in structure, and are usually called microbes by optical microscopy and electron microscopy. They include prokaryotic microorganisms such as bacteria, actinomycetes, rickettsia, mycoplasma, chlamydia, cyanobacteria, and also eukaryotic microorganisms such as yeasts, enzymes, protozoa, microalgae, and non-cell type viruses and Ill. Therefore, microbes are not a taxonomic concept, but a general term for all tiny organisms.

Common microorganisms include: bacteria, fungi, spores, viruses, and the like.

The importance of space disinfection

As an important channel for microbial transmission, space disinfection is one of the important links in all environmental disinfection. It is a disinfection link that must be controlled by various enterprises and institutions and related production enterprises (pharmaceutical industry, food industry). China's new GMP Pharmacopoeia clearly specifies the number of floating bacteria in the space. It can be seen that spatial disinfection plays a decisive role in the transmission of cut microorganisms.

Specific methods of space disinfection:

The current common methods of space disinfection are:

1, ozone fumigation space disinfection:

Ozone is a strong oxidant and the sterilization process is a biochemical oxidation reaction. Ozone sterilization has the following three forms:

1. Ozone can oxidize and decompose the enzymes required for glucose inside the bacteria, causing the bacteria to inactivate and die.

2 Directly interact with bacteria and viruses, destroying their organelles and DNA, RNA, and destroying the metabolism of bacteria, leading to bacterial death.

3 through the cell membrane tissue, invading the cells, acting on the outer membrane of lipoproteins and internal lipopolysaccharide, causing the bacteria to undergo permeability distortion and dissolve and die.

2, formaldehyde aerosol fumigation space disinfection:

1 Calculated according to formaldehyde (40%) 10 ml / cubic meter, potassium permanganate 5 g / cubic meter. In different cases, the dosage is different, but the ratio is 2:1.

2 The container is large, heat-resistant and corrosion-resistant: ceramic or glass containers are generally used, because potassium permanganate and formaldehyde are corrosive, and the reaction is intense after mixing, releasing heat.

3 rooms should be sealed: the fumigation effect will be good.

4 The container should be as close as possible to the door so that the operator can quickly evacuate; firstly pour warm water into the container, then add and mix evenly; then add formaldehyde; after adding formaldehyde, the person immediately leaves and closes the room. Note the order: Pour formaldehyde into the potassium permanganate solution.

5 disinfection time is generally 20-30 minutes.

6 After disinfection, open the doors and windows to ventilate. The formaldehyde here is 40% formaldehyde solution, which is formalin. The general packaging is 37%.

3, electric aerosol sprayer space disinfection.

Aerosol sprayer is a new multi-purpose spray disinfection device. It uses a double cyclone airflow atomizing nozzle and a medicine bottle to form a spray component. The electric centrifugal fan and the base form a power component. The spray hose is connected to the power component by a corrugated hose. Composed together.

The aerosol sprayer is made of corrosion-resistant engineering plastics with high atomization efficiency, good performance, concentrated particle spectrum, and can spray disinfection and sterilization preparations with good oxidation resistance (such as hydrogen peroxide). Especially suitable for health and epidemic prevention departments (such as hospitals, epidemic prevention stations, disinfection stations), tourist hotels, kindergartens, schools, institutions canteens, food processing places, various farms (such as animal breeding houses), stations, docks, warehouses, aircraft Disinfection, sterilization, insecticide, deodorization, humidification, etc. of indoor conditions such as ships, vehicles, and homes.

4, peroxide disinfection (HPV) technology space disinfection:

The technique converts liquid H2O2 into HPV by "flashing", and the process can be effectively carried out in a normal temperature and normal humidity environment, so that special pretreatment such as dehumidification is not required.

The HPV is uniformly introduced into the confined space, and the inner surface thereof is completely exposed to the HPV, and a hydrogen peroxide film of about 1 micrometer is formed to adhere to the surface of the microorganism that may be colonized, and the microorganism itself is wrapped as microcondensation formed by the core, and is quickly Kill. After the disinfection, the HPV is catalytically decomposed into water vapor and oxygen. It can also be completely decomposed by using a powerful ventilation device, or a building air conditioning ventilation system can be used. For the freeze dryer, the vacuum system can be used to quickly remove residual HPV.

5, dry mist hydrogen peroxide silver ion sporicide space disinfection:

Silver ion peroxide sporicide is a strong oxidant. The released oxygen molecules (primary oxygen) can penetrate and destroy the cell wall and cell membrane of the microorganism, and enter the cell to make its key functional components (such as DNA and enzyme). Oxidation of the enzyme system of microorganisms impedes the basic metabolic functions of bacteria (enzymes) or affects their membrane structure leading to cell death. The destruction of all components in the microbial cells is irreversible, which ensures the thoroughness of the sterilization and, more importantly, because of this unique mechanism, the microorganisms are not resistant. Because of its unique characteristics of high sterilization efficiency, safe residue, colorless, odorless and non-toxic, silver ion peroxide sporicide is widely used in practical production. Under the general environment that EU pharmaceutical companies have completely banned formaldehyde, dry atomized hydrogen peroxide silver ion sporicide is currently the first choice for many EU pharmaceutical companies. It is a new attempt in space disinfection and represents a new direction in the development of space disinfection.

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