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The bottom of the Test Tube, which is to be stored at the bottom of the well, is sealed with a sterile rubber plug and paraffin, and then it is sealed in a sealed wide-mouth bottle or plastic bag. It is submerged in the bottom hole and can be stored for 2 to 5 months.
Paraffin sealing storage Cut the wax paper into 6 to 8 cm squares and soak it in 75% alcohol for 1 minute. After the mouth of the tube species that has been cultured is rotated and sterilized above the flame of the alcohol lamp, the wax paper is taken out with tweezers and the nozzle is tightened. The stenciled paper nozzle is then immersed in molten paraffin, and the wax paper is completely immersed so that the wax paper is tightly packed on the wall of the tube and no air leakage occurs. After the paraffin cools and solidifies, it is stored in the refrigerator or at room temperature.
Cellophane sealed gelatin 15 grams (binder), copper sulfate 2 grams, 1 to 2 drops of methylene blue (indicator), dissolved in 100 ml of hot water, formulated as a sealing agent. Remove the newly grown mother-of-pearl tampon, face down, place a sealing agent, attach cellophane soaked in 95% alcohol, and seal with paraffin wax. At room temperature, strains can be stored for at least 8 months and can prevent the infestation of molds or maggots. Injecting sterile mineral oil (liquid paraffin) on the slope can greatly extend the storage time.
Manure storage This method is suitable for storage of grass mushroom and other rot fungi. Combine composted manure and dried and broken into pieces, take 300g of dry compost, sieve with a sieve of 5mm in diameter, add 1000ml of water to adjust the humidity, and put it into a test tube, taking up 1/3 to 1/2 of the depth of the tube, at 0.15 2 hrs under MPa pressure. After confirmation of asepsis, inoculation, after the mycelium is full, it is stored in a refrigerator at 3°C ​​and transplanted every two years. In long-term storage, if the medium loses water, the storage bacteria should be inoculated on the agar slant for activation, and then continue to save. After this method was continuously stored for 4 years, its production traits did not change significantly.
The preservation of edible mushroom strains can effectively maintain its superior species and extend the years of use of the fine varieties. Therefore, it is of equal importance to the selection and breeding of new varieties and is an indispensable and important link in the production of edible fungi. However, formal and rigorous storage methods require some equipment. They also have high requirements for operating techniques. For the majority of mushroom farmers, some simple soil methods are more feasible and more popular.