Correct understanding and advice on ozone air disinfection In gas phase conditions, the effect of ozone killing microorganisms depends on several major factors: the concentration of ozone threshold, that is, when the ozone air concentration reaches this concentration requirement, it can have the ability to kill microorganisms; microbial species; processing time; Ozone distribution methods; temperature and humidity conditions in space; reductive effects of interior wall surfaces, ceilings, floors, and other decorative materials.
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Under gas phase conditions, the killing effect of ozone on microorganisms is different. It has been experimentally proven that ozone has a strong bacteriolytic effect against human and animal pathogenic bacteria and viruses.
The environmental factors affecting the bactericidal effect of gas phase ozone are mainly temperature and humidity. Under normal circumstances, a low temperature, high humidity and good killing effect, relative humidity> 70%, the killing effect can be well reflected. This is due to the increase in relative humidity, which allows the cells to swell and the cell walls to become thinner, making them more susceptible to the osmotic solubilization of ozone.
Ozone toxicity, corrosion and safety explained: Ozone is a non-toxic substance safety gas, speaking of its toxicity is mainly its strong oxidizing ability, when the concentration is higher than 1.5ppm, the personnel must leave the scene because of ozone stimulation The respiratory system, which causes a stressful reaction in the respiratory system, can cause serious reversible harm. For this reason, the International Ozone Association (IOA) has developed a health standard: International Ozone Association: 0.1ppm, exposure to 10 hours; US: 0.1ppm, Exposure to 8 hours; China: 0.15 ppm, 8 hours of exposure The literature reports that when the ozone concentration is 0.02 ppm, people with olfactory sensations can perceive it as a sensory critical value. When the concentration is 0.15 ppm, it is the olfactory threshold, and most people can smell it. Out, it is also a health standard point. When the concentration reaches 1-10 ppm, it is called the stimulation range. In fact, the safe use of ozone can completely ensure that people's health is not compromised. In the past more than one hundred years since the application of ozone, there has been no case of accidental death due to ozone poisoning in the world.
In the specific use, the ozone disinfection process should be isolated from the personnel, the general ozone generating device is automatically controlled, as long as the relevant parameters are set, that can achieve unattended, which requires specialized training with chemical disinfectant spray disinfection. Compared with the on-site operations, the personnel's health is more assured.
Ozone is highly oxidizing. Ozone can oxidize many metals. Contact with aluminum, zinc, lead and ozone can cause oxidative corrosion, but chromium-iron alloys (stainless steel) containing 25% Cr are basically not subject to ozone corrosion.
Ozone has the greatest effect on the corrosion of ordinary rubber. Therefore, corrosion-resistant silicone rubber or rubber products with added corrosion inhibitors should be used in applications.
Ozone generation is generally powered by high-voltage power supply, so the use of conductive gases or hydrocarbon-containing media should be eliminated, and personnel must perform the procedures safely.
Based on the above factors, we recommend the use of ozone generators for air disinfection:
Air disinfection with ozone generator installation. Split or mobile in a separate space, usually placed high, because the proportion of ozone in the air is larger, easy to drop, the purpose of placing a high place is mainly conducive to the spread of ozone; in relatively closed space, such as air The self-circulating sterile room, clean room and special requirements space are recommended to be used in closed-circuit air circulation systems, adding ozone generating devices, such as assembled in the air conditioning unit, installed in the total air supply duct or the total return air duct, to reach the design After the disinfection time is fixed, air-sterilization and sterilization are performed using activated carbon adsorption or catalyst reduction. This can not only achieve the purpose of air disinfection, but also enable the staff to leave the work environment. At present, the application of the technology in the sterile room of the pharmaceutical industry has been very extensive and mature. The main workflow is as follows: air compressor - oxygen generator - ozone generator - air conditioning total air supply pipe - the disinfection and purification zone.
Air disinfection using ozone generator device selection calculations. The use of ozone disinfection needs to reach at least the threshold concentration. If the concentration is lower than the threshold concentration, the sterilization effect cannot be achieved, and if the concentration is too high, the operating cost will increase. Therefore, it should be calculated that the selected range of occurrence concentration of ozone occurs. Device. According to the “Disinfection Technical Specifications†and practical application data, in the clean area of ​​the GMP pharmaceutical workshop, the cleanliness level of 300,000 levels need to keep ozone concentration N=5mg/M3, and 100,000 grades take N=10 mg/M3. Take N=30mg/M3, and take N=40mg/M3 in one hundred steps. The formula for calculating the total amount of ozone is: W=NV/(1-S), where W-ozone total dosage (mg/M3); S-ozone decay rate S=40%; V-total volume (M3); N - The concentration of ozone (mg/M3) to be maintained in the clean area. Where V = V1 + V2 + V3, V1 - the total volume of the clean area; V2 - the volume of the air-conditioning duct; V3 - the effective volume of the ozone loss caused by the new volume of fresh air (V3 = 20% of the total air volume; 10% of the normal fresh air replacement rate (maintaining positive pressure in the clean area) Supplementary fresh air volume) 60% = Total air volume 1.2%).
The humidity is appropriate. The sterilization effect of ozone is best under the condition of humidity of 70-80%, and the effect is poor when the humidity is lower than 45%, so in general use, especially in the sterile room and ward of pharmaceutical workshops, attention should be paid in the environment. Increase humidity properly.
Ozone concentration. When using ozone for air disinfection, it should be noted that the bactericidal effect of ozone K is the product of the actual concentration of ozone C and the action time T, that is, K=CT. ​​When the actual concentration of ozone C reaches the bactericidal threshold concentration Cmin, even if the time is extended, it is actually In operation, I am afraid there is no bactericidal effect. Ozone is used for air disinfection. Ozone generators with different ozone production and ozone generation concentrations should be configured according to different application spaces and different sterilization purposes. Ozone generation concentration refers to the concentration of 30 minutes within a fixed volume by means of natural diffusion. The unit is mg/m3. Ozone production is calculated from the ozone concentration and time. Ozone production and ozone concentration are the most basic parameters. Therefore, ozone disinfection products for the purpose of air disinfection should be marked with the disinfection concentration and disinfection time indicators. There are also two general indicators, namely, ozone safety indicators for application space and product lifetime indicators, which are also required for users. As for the general deodorization, deodorization, etc., the concentration is generally not more than 0.05ppm. If used for indoor sterilization, it is generally controlled at 0.1-1ppm; the surface of the object or the ward area generally requires higher concentrations.
Air disinfection ozone generator selection. Because ozone can only be produced locally, the most economical and technologically mature method for producing ozone is corona discharge, and the ozone generator used for air disinfection is mostly a ceramic plate high-frequency discharge mode, which has a simple structure and performance. Reliable, low operating costs and flexible adjustments.
Ozone air disinfection safety. Ozone Safety In a wide range of animal and human trials, the main route of exposure is inhalation, and sustained, long-term inhalation tests have shown that ozone is not cancerous (NTP, 1995); but there is a clear stimulatory effect on the respiratory system of animals and humans. In an exposed environment, respiratory function may be affected. Therefore, we suggest that the use of ozone to disinfect the air should be conducted in an unmanned environment, and personnel should be allowed to enter after half an hour of disinfection. 
